Gentaur offers the fastest delivery of selected and validated reagents. If the product doesn’t detect your biological sample we will try to trouble shoot your protocol or offer money back guarantee.
What are antibodies used for? Anti-geneantibodies are used in Western Blot, Ihc (Immuno-histo-chemistry), flow (facs) and in immunoprecipitation to recognize an epitope of your target protein.
How most antibodies are stored? Most are stored at -20°C with 0.5% of sodium azide (NaN3). The azide makes them even stable at room temperature. However the azide is quiet sticky and on request Gentaur offers azide free antibodies.
What are Monoclonal antibodies? These are antibodies produced in a hybridoma cell line that is obtained from the fusion of a mouse splenocyte (B-lymphocyte) and permanent mouse cell line. Most monoclonal are made from mouse cells but also, rat, rabbit, camel and shark antibodies are popular.
Why is aMousemonoclonal antibody is more reliable than a Rabbit polyclonal antibody? The hybridoma or clone to produce monoclonals is always the same clone but the rabbits that are used are different even if the injected antigen or epitope is the same. How large is an epitope to produce antibodies? The synthetic protein is between 15 and 45 amino acids and is chosen on a c-terminal or n-terminal site of the protein because there is more chance these are at the outside of the protein.
Why Klh is added to produce polyclonal antibodies? Klh (Keyhole limpet hemocyanin) is a strong antigenicity triggering agent that is quantitative and qualitative standardized.
A selection audit will help you to find the rightFitclabelled monoclonal for flow cytometry, antibodies,dna or IgG antibody. How is a Blocking peptide used? Detecting antigens with antibodies can only be confirmed if you block the antibody with an added antigen and the signal or band disappears in your next Western Blot.